Molecular cloning and functional analyses of glutathione peroxidase homologous genes from Chlorella sp. NJ-18

Article ID	Journal	Published Year	Pages	File Type
5907352	Gene	2012	7 Pages	PDF

Abstract

âº We identify two GPX homologous genes in Chlorella. âº We examine alternative splicing of their transcripts. âº We examine differential responses of GPX genes to oxidative stresses. âº The purified recombinant proteins show thioredoxin-dependent peroxidase activities. âº Expression of a GPX gene in Synechocystis enhances its oxidative tolerance.

Keywords

SDS ORF IPTG NADPH PAGE RT-PCR kDa kiloDalton 5′ RACE cDNA Complementary DNA amino acid residue(s)Ultraviolet polyacrylamide gel electrophoresis isopropyl β-D-thiogalactopyranoside Induced expression Base pair(s)Min minute Hour sodium dodecyl sulfate Synechocystis open reading frame UTR یا untranslated regions untranslated region Isoelectric point Nucleotide nicotinamide adenine dinucleotide phosphate reverse-transcription polymerase chain reaction threshold cycle Chlorella kilobase(s)