Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5907423 | Gene | 2012 | 11 Pages |
Testis-specific tandemly repeated Stellate genes are part of the Ste-Su(Ste) genetic system required for male fertility in Drosophila melanogaster. Stellate genes encode a functional homolog of the β-subunit of protein kinase CK2. Derepression of Stellate results in their over-expression, meiotic disturbances and male sterility. Stellate genes are represented by clustered copies in the X chromosome and carry promoters shared with another X-chromosome cluster, βNACtes genes, encoding putative β-subunits of the nascent polypeptide-associated complex. Using Electrophoretic Mobility Shift Assay, we revealed in the Stellate promoter three cis-acting elements, E-boxes, the loss of which greatly diminished the reporter gene expression in Drosophila testes. We identified that these E-boxes were recognized by helix-loop-helix protein, dUSF (Drosophila ortholog of mammalian USF) in testis nuclear extract. All three E-boxes were preserved in the promoters of both euchromatic and heterochromatic Stellate clusters. Two analogous E-boxes were detected in the promoters of 5â²-copies of the duplicated βNACtes gene pairs, whereas the 3â²-copies lacked these sites but possessed a new binding site for a testis protein distinct from dUSF. Here we characterized a new type of testis-specific core promoter and identified dUSF as its interacting transcription factor.
⺠Regulation of Ste-Su(Ste) system in Drosophila spermatogenesis. ⺠Cis-regulatory elements, E-boxes, were found in Stellate promoter. ⺠E-box-binding transcription factor dUSF is expressed in the testes. ⺠Ste-Su(Ste) and βNACtes genes employ common regulatory elements in shared promoters. ⺠A new type of testis-specific promoter was described.