A subset of human gliomas shows over-expression of KIT without its amplification

Article ID	Journal	Published Year	Pages	File Type
5907472	Gene	2012	9 Pages	PDF

Abstract

âº Altered KIT expression in gliomas is independent of gene amplification. âº KIT expression is associated with transcriptional up-regulation. âº KIT over-expression in gliomas occurs across low/high grades. âº Most KIT immunopositive gliomas co-express receptor and ligand (KITLG) transcripts.

Keywords

GBM Oligodendroglioma GAPDH HRP ΔCT PBLs SEGA AOA mRNA PDGFR RT-PCR NTC Anaplastic oligoastrocytoma GISTs SDS 3, 3′-Diaminobenzidine RTC PDGF RPO qPCR DAB RNase P 6-diamidino-2-phenylindole BSA cDNA Complementary DNA DNA BAC FFPE RTKs cycle threshold Anaplastic astrocytoma Diffuse astrocytoma pilocytic astrocytoma bovine serum albumin EDTA Ethylenediaminetetraacetic acid deoxyribonucleic acid RNA ribonucleic acid Anaplastic oligodendroglioma IHC Immunohistochemistry HIER beta-actin No template control Relative expression copy number variation sequencing CNS messenger ribonucleic acid ribonuclease P Female World Health Organization sodium dodecyl sulfate central nervous system coefficient of determination Actb platelet-derived growth factor formalin fixed paraffin embedded fluorescence in situ hybridization peripheral blood leucocytes Fish Relative quantitation Male Chromogenic in situ hybridization reverse transcriptase polymerase chain reaction polymerase chain reaction quantitative real-time PCR PCR Horseradish peroxidase gene of interest bacterial artificial chromosome WHO CISH glyceraldehyde 3-phosphate dehydrogenase Glioblastoma multiforme receptor tyrosine kinases platelet-derived growth factor receptor