Negative regulation of human U6 snRNA promoter by p38 kinase through Oct-1

Article ID	Journal	Published Year	Pages	File Type
5907483	Gene	2012	8 Pages	PDF

Abstract

âº p38 kinase inhibitors, SB202190 and SB203580, enhanced transcription of U6 promoter. âº Stimulation of U6 promoter activity by SB202190 is dependent on octamer sequence. âº SB202190-treated nuclear extract showed increased level of octamer binding activity. âº p38 kinase inhibitor restored down-regulation of U6 promoter by stress, PMA or p38Î±. âº Cellular U6 RNA was augmented in SB202190-treated Jurkat cells.

Keywords

phorbol 12-myristate 13-acetate DSE TBE DNA-PKcs JNKs ΔΔCt pKa PAGE qRT-PCR RPMI-1640 snRNA GAPDH SNAPc p38 kinase PI3K PBS Roswell Park Memorial Institute medium 1640 DMEM SDS c-Jun amino-terminal kinases IgG SB202190 HEPES cDNA DMSO DNA complementary to RNA Dulbecco's modified Eagle Medium ERKs PMA MAPK small nuclear RNA RNA polymerase III Electrophoretic mobility shift assay STAT EDTA Ethylenediaminetetraacetic acid polyacrylamide gel electrophoresis Oct-1 immunoglobulin G Bis-tris Tris-Borate-EDTA base pair Dimethylsulfoxide real-time quantitative PCR sodium dodecyl sulfate EMSA یا electrophoretic mobility shift assay distal sequence element proximal sequence element phosphoinositide 3-kinase Infrared signal transducers and activators of transcription Phosphate-buffered saline MeS DNA-dependent protein kinase catalytic subunit polymerase chain reaction PCR protein kinase A mitogen-activated protein kinase U6 promoter PSE Geneticin extracellular signal-regulated kinases Gas glyceraldehyde-3-phosphate dehydrogenase