| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5907546 | Gene | 2012 | 7 Pages |
Abstract
⺠Bacillus anthracis is poorly transformed by DNA methylated on adenine or cytosine. ⺠We inactivated three genes encoding methylation-dependent restriction enzymes. ⺠The resulting strains showed increased transformation by methylated DNA. ⺠The genes mrr, mcrBP, and mcrB3P each contribute to the restriction of cytosine-methylated DNA. ⺠Adenine methylation may cause inefficient replication of hemimethylated plasmid DNA.
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Authors
Ramakrishnan Sitaraman, Stephen H. Leppla,