Structural features of cytochrome P450 1A associated with the absence of EROD activity in liver of the loricariid catfish Pterygoplichthys sp.

Article ID	Journal	Published Year	Pages	File Type
5907587	Gene	2011	8 Pages	PDF

Abstract

âº EROD was not detected in Pterygoplichthys liver microsomes âº CYP1A protein and mRNA were induced by treatments with AhR agonists. âº CYP1A coding region exhibits six exclusive amino acids substitutions. âº EROD substrate docked infrequently and in an unusual position at the active site.

Keywords

RMSD CYP1A EROD i.p.DMBA RMS BNF ethoxyresorufin-O-deethylase NADPH SRS PDB RMSF PCB126 ITS TRIS qPCR 7,12-dimethylbenz[α]anthracene AHR DMSO Internal transcribed spacer amino acid Ethoxyresorufin Root-mean-squared deviation Tris(hydroxymethyl)aminomethane Substrate specificity Intraperitoneally Molecular dynamics Dimethyl sulfoxide quantitative real time PCR Biotransformation substrate recognition site cytochrome P450 1a root mean square polymerase chain reaction PCR Protein Data Bank optical density reduced nicotinamide adenine dinucleotide phosphate aryl hydrocarbon receptor