Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5907618 | Gene | 2010 | 12 Pages |
Abstract
The cp27 gene is a highly conserved and unique gene with important roles related to craniofacial organogenesis. The present study is a first analysis of the CP27 promoter and its regulation. Here, we have cloned the promoter of the mouse cp27 gene, examined its transcriptional activity, and identified transcription factor binding sites in the proximal promoter region. Two major transcription start sites were mapped adjacent to exon 1. Promoter function analysis of the 5â² flanking region by progressive 5â² deletion mutations localized transcription repression elements between â1993Â bp and â969Â bp and several positive elements between â968Â bp and the preferred transcription start site. EMSA and functional studies indicated two function-cooperative CCAAT boxes and identified the NF-Y transcription factor as the CCAAT activator controlling transactivation of the CP27 promoter. In addition, this study demonstrated that for its effective binding and function, NF-Y required not only the minimal DNA segment length identified by deletion studies, but also a defined nucleotide sequence in the distal 3â² flanking region of the CP27 proximal promoter CCAAT box. These results provide a basis for our understanding of the specific regulation of the cp27 gene in the NF-Y-mediated gene transcription network.
Keywords
FGFRNA ligase mediated rapid amplification of cDNA endsCdc25 phosphataseNF-1NF-YHSP 70Cdc25cAMVRLM-RACEETsAP-1CBFC/EBPSOxPOUElectrophoretic mobility shift assayGATAOctPromoter structureEMSA یا electrophoretic mobility shift assay nuclear factor 1Nuclear factor Yfibroblast growth factorCore binding factoravian myeloblastosis virusCCAAT/enhancer binding proteinheat shock protein 70
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Genetics
Authors
Xianghong Luan, Yoshihiro Ito, Youbin Zhang, Thomas G.H. Diekwisch,