Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5907809 | Genomics | 2013 | 7 Pages |
Abstract
We reported HIVID (high-throughput Viral Integration Detection), a novel experimental and computational method to detect the location of Hepatitis B Virus (HBV) integration breakpoints in Hepatocellular Carcinoma (HCC) genome. In this method, the fragments with HBV sequence were enriched by a set of HBV probes and then processed to high-throughput sequencing. In order to evaluate the performance of HIVID, we compared the results of HIVID with that of whole genome sequencing method (WGS) in 28 HCC tumors. We detected a total of 246 HBV integration breakpoints in HCC genome, 113 out of which were within 400Â bp upstream or downstream of 125 breakpoints identified by WGS method, covering 89.3% (125/140) of total breakpoints. The integration was located in the gene TERT, MLL4, and CCNE1. In addition, we discovered 133 novel breakpoints missed by WGS method, with 66.7% (10/15) of validation rate. Our study shows HIVID is a cost-effective methodology with high specificity and sensitivity to identify viral integration in human genome.
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Authors
Weiyang Li, Xi Zeng, Nikki P. Lee, Xiao Liu, Shengpei Chen, Bing Guo, Shang Yi, Xuehan Zhuang, Fang Chen, Guan Wang, Ronnie T. Poon, Sheung Tat Fan, Mao Mao, Yingrui Li, Songgang Li, Jun Wang, JianWang JianWang, Xun Xu, Xiuqing Zhang,