Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5907821 | Genomics | 2013 | 7 Pages |
Abstract
The native Japanese cattle Mishima-Ushi, a designated national natural treasure, are bred on a remote island, which has resulted in the conservation of their genealogy. We examined the genetic characteristics of 8 Mishima-Ushi individuals by using single nucleotide polymorphisms (SNPs), insertions, and deletions obtained by whole-genome sequencing. Mapping analysis with various criteria showed that predicted heterozygous SNPs were more prevalent than predicted homozygous SNPs in the exonic region, especially non-synonymous SNPs. From the identified 6.54Â million polymorphisms, we found 400 non-synonymous SNPs in 313 genes specific to each of the 8 Mishima-Ushi individuals. Additionally, 3,170,833 polymorphisms were found between the 8 Mishima-Ushi individuals. Phylogenetic analysis confirmed that the Mishima-Ushi population diverged from another strain of Japanese cattle. This study provides a framework for further genetic studies of Mishima-Ushi and research on the function of SNP-containing genes as well as understanding the genetic relationship between the domestic and native Japanese cattle breeds.
Keywords
CFTRNon-synonymous SNPsBACSUTRsGAAQTLSNPsMitochondrial DNAnsSNPsmolecular evolutionary genetics analysisBos taurusNext-generation sequencinginsertions and deletionsmtDNAcystic fibrosis transmembrane conductance regulatorquantitative trait locusCASTUntranslated regionsMEGAGene ontologyIndelspolymerase chain reactionPCRSingle-nucleotide polymorphismsneighbor-joiningbacterial artificial chromosomesCalpastatin
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Authors
Kaoru Tsuda, Ryouka Kawahara-Miki, Satoshi Sano, Misaki Imai, Tatsuo Noguchi, Yousuke Inayoshi, Tomohiro Kono,