Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5908039 | Genomics | 2006 | 12 Pages |
Abstract
To identify the disease-susceptibility genes of type 2 diabetes, we performed quantitative trait loci (QTL) analysis in F2 populations generated from a BKS.Cg-m+/+Leprdb and C3H/HeJ intercross, taking advantage of genetically determined obesity and diabetes traits associated with the db gene. A genome-wide scan in the F2 populations divided by sex and db genotypes identified 14 QTLs in total and 3 major QTLs on chromosome (Chr) 3 (LOD 5.78) for fat pad weight, Chr 15 (LOD 6.64) for body weight, and Chr 16 (LOD 8.15) for blood glucose concentrations. A linear-model-based genome scan using interactive covariates allowed us to consider sex- or sex-by db-specific effects of each locus. For the most significant QTL on Chr 16, the high-resolution haplotype comparison between BKS and C3H strains reduced the critical QTL interval from 20 to 4.6Â Mb by excluding shared haplotype regions and identified 11 nonsynonymous single-nucleotide polymorphisms in six candidate genes.
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Authors
Maki Moritani, Katsuhiko Togawa, Hiroshi Yaguchi, Yuka Fujita, Yuka Yamaguchi, Hiroshi Inoue, Naoyuki Kamatani, Mitsuo Itakura,