Article ID Journal Published Year Pages File Type
5912915 Plasmid 2012 11 Pages PDF
Abstract
► We isolated mutations in the Vibrio cholerae chromosome II replication initiation protein RctB. ► All mutations gave a copy-up phenotype in a minichromosome based system in Escherichia coli. ► All mutations decreased the RctB-RctB interaction. ► All mutations influenced rctB autoregulation. ► The maximal C-terminal deletion giving a functional RctB was165 amino acids.
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Life Sciences Biochemistry, Genetics and Molecular Biology Genetics
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rctB mutations that increase copy number of Vibrio cholerae oriCII in Escherichia coli
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