Article ID Journal Published Year Pages File Type
5912934 Plasmid 2013 8 Pages PDF
Abstract
► We constructed a lentiviral Tet On overexpression plasmid controlled by aP2 promoter. ► The combination of TRE + rtTAadv in vectors resulted in the lowest basal expression. ► TRE + rtTAadv also gave the best inducibility by Dox in HIB-1B and 3T3-L1 cell lines. ► A truncated aP2 promoter conferred adipogenic expression in HIB-1B cells.
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Life Sciences Biochemistry, Genetics and Molecular Biology Genetics
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