Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5912934 | Plasmid | 2013 | 8 Pages |
Abstract
⺠We constructed a lentiviral Tet On overexpression plasmid controlled by aP2 promoter. ⺠The combination of TRE + rtTAadv in vectors resulted in the lowest basal expression. ⺠TRE + rtTAadv also gave the best inducibility by Dox in HIB-1B and 3T3-L1 cell lines. ⺠A truncated aP2 promoter conferred adipogenic expression in HIB-1B cells.
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Authors
Q. Liu, P.J. Hill, A. Karamitri, K.J.P. Ryan, H.Y. Chen, M.A. Lomax,