Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5913830 | Journal of Structural Biology | 2015 | 9 Pages |
Abstract
In early 2011 FEI Company launched the “Falcon”, its first commercial direct electron detector product intended for application in 3-D electron microscopy in the life sciences. In this paper we discuss the principle of direct electron detection and its implementation in Falcon cameras. We describe the signal formation in the sensor and its impact on the detection quantum efficiency (DQE) of the sensor. Insights into the signal formation led us to improved camera designs. Three significant improvements are discussed. (1) Back thinning of the sensor. This is implemented in the second-generation Falcon (Falcon 2), where the sensor thickness is reduced to 50 μm, and in the latest generation Falcon 3 detector with further back-thinning down to 30 μm. (2) The introduction of electron counting, a signal processing technology implemented in Falcon 3. (3) Dose fractionation mode, which allows the user to access intermediate results during the illumination of the sample.
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Authors
Maarten Kuijper, Gerald van Hoften, Bart Janssen, Rudolf Geurink, Sacha De Carlo, Matthijn Vos, Gijs van Duinen, Bart van Haeringen, Marc Storms,