Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5914925 | Journal of Structural Biology | 2010 | 11 Pages |
Abstract
With the CNB, high-pressure frozen samples can be milled to produce either thin lamellas (<100Â nm), for direct imaging by high-resolution cryo-TEM or thicker lamellas (300-500Â nm) for cryo-electron tomography. The sample remains vitreous throughout the process by using the presented tools and methods. The results are an important step towards investigating larger cells and even tissue in there natural state which in the end will enable us to gain better insights into cellular processes.
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Authors
Michael F. Hayles, D.A. Matthijs de Winter, Chris T.W.M. Schneijdenberg, Johannes D. Meeldijk, Uwe Luecken, Hans Persoon, Jeroen de Water, Frank de Jong, Bruno M. Humbel, Arie J. Verkleij,