Article ID Journal Published Year Pages File Type
5914925 Journal of Structural Biology 2010 11 Pages PDF
Abstract
With the CNB, high-pressure frozen samples can be milled to produce either thin lamellas (<100 nm), for direct imaging by high-resolution cryo-TEM or thicker lamellas (300-500 nm) for cryo-electron tomography. The sample remains vitreous throughout the process by using the presented tools and methods. The results are an important step towards investigating larger cells and even tissue in there natural state which in the end will enable us to gain better insights into cellular processes.
Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Molecular Biology
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