Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5915276 | Molecular and Biochemical Parasitology | 2016 | 14 Pages |
â¢Discovery of the kDNA maxi-circle.â¢Discovery of the glycosome.â¢Discovery of the mechanism of tryp antigenic variation.â¢Discovery of DNA base J.â¢Discovery of the function of base J as a transcriptional terminator in Leishmania.
This is a personal story of the author of his research on trypanosomatids, covering a period of 1970-2015. Some of the highlights include the discovery of new aspects of kDNA, the mini-circle heterogeneity and the maxi-circle; the glycosome; the discovery of gene transposition as a major mechanism for antigenic variation; trans-splicing as an essential step in the synthesis of all trypanosome mRNAs; Pulsed Field Gradient gels to size-fractionate chromosome-sized DNA molecules of protozoa; the sequence of trypanosome telomeres and their growth and contraction; the first ABC-transporter of trypanosomatids, LtpgpA; the variable transferrin receptor of T. brucei and its role in Fe uptake; and base J, its structure, biosynthesis and function.