| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5915550 | Molecular and Biochemical Parasitology | 2012 | 11 Pages |
Abstract
⺠A recombinant Toxoplasma gondii dihydroorotate dehydrogenase (TgDHOD) was kinetically characterized. ⺠TgDHOD was not significantly affected by known inhibitors of human DHOD. ⺠Three Plasmodium falciparum DHOD inhibitors were tested and exhibited one to two orders of magnitude higher IC50s for TgDHOD. ⺠C-terminally tagged TgDHODs in transgenic T. gondii colocalize with the parasite's mitochondrion. ⺠Gene targeting suggested an essential role for TgDHOD that was not rescued by uracil supplementation.
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Authors
Miryam Andrea Hortua Triana, My-Hang Huynh, Manuel F. Garavito, Barbara A. Fox, David J. Bzik, Vern B. Carruthers, Monika Löffler, Barbara H. Zimmermann,