Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5915819 | Molecular and Biochemical Parasitology | 2010 | 9 Pages |
We isolated a galectin-9 (Gal-9) homologue gene (Tl-gal) from an adult worm of the canine gastrointestinal nematode parasite, Toxascaris leonina, via random cDNA library sequencing. The deduced amino acid sequence of the Tl-gal genes evidenced an identity of 89% with the galectin of Dirofilaria immitis, 87% identity with the galectin of Brugia malayi, and 35% identity with the human GAL-9 gene. To evaluate immune modulate function of Tl-GAL in host inflammatory response, we constructed recombinant Tl-GAL (rTl-GAL) using an Escherichia coli expression vector system and treated to intestinal inflammation mice. Although the carbohydrate-binding ability of rTl-GAL was less than that of rat galectin, we confirmed that recombinant rTl-GAL has carbohydrate-binding activity. The clinical symptoms of dextran sulfate sodium (DSS)-treated mice after rTl-GAL pre-treatment were found to be minimized, or less profound, as compared to those of the rTl-GAL untreated group. Additionally, the DSS-treated mice exhibited a significant shortening of the colon, but the large intestines of the rTl-GAL pre-treated mice were longer than those of the control group (P < 0.05). Additionally, the rTl-GAL treated group exhibited significantly increased the levels of TGF-β and IL-10 (P < 0.05). The production of these regulatory cytokines may ameliorate intestinal inflammation. These findings demonstrate that rTl-GAL could inhibit inflammation reactions via the inhibition of Th1 and Th2 cytokine production by increasing the production of TGF-β and IL-10 cytokines. The rTl-GAL may induce TGF-β expression, primarily via the activation of the p38 pathway. In conclusion, rTl-GAL may function like a host galectin, thus functioning as a regulatory molecule in the host immune system; rTl-GAL may prove useful in the design of novel therapeutic intervention strategies for the treatment of allergic and immune diseases.
Graphical abstractWe found some interesting things about galectin homologues (Tl-GAL) from intestinal parasite Toxascaris leonina. Recombinant Tl-GAL (rTl-GAL) inhibited dextran sulfate sodium-induced intestinal inflammation by IL-10 and TGF-β cytokine expression.Download high-res image (108KB)Download full-size image