A family of cathepsin F cysteine proteases of Clonorchis sinensis is the major secreted proteins that are expressed in the intestine of the parasite

Localizations of CsCF-4, CsCF-6, and CsCF-11. (A) Sections of Clonorchis sinensis adult worms were probed with rat anti-CsCF-4 and mouse anti-CsCF-6 followed by FITC-conjugated anti-rat IgG and TRITC-conjugated anti-mouse IgG. The slide was observed with a confocal laser scanning microscope (10× magnification). (B) Sections of C. sinensis adult worms were probed with mouse anti-CsCF-6 and rat anti-CsCF-11 followed by FITC-conjugated anti-rat IgG and TRITC-conjugated anti-mouse IgG. The slide was observed with a confocal laser scanning microscope (100× magnification). I, intestine; L, intestinal lumen; U, uterus; SR, seminal receptacle. Scale bar indicated 100 μm.