Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5916938 | Molecular Immunology | 2013 | 11 Pages |
Abstract
Following uptake and subsequent presentation of avian influenza virus by DCs, adaptive immunity, including humoral immune responses are induced. We have investigated the antibody responses against virus glycan epitopes after avian influenza virus infection. Using glycan micro-array analysis we showed that chicken contained antibodies that predominantly recognize terminal Galα1-3Gal-R, chitotriose and Fucα1-2Galβ1-4GlcNAc-R (H-type 2). After influenza-infection, glycan array analysis showed that both levels and repertoire of glycan-recognizing antibodies decreased. However, analysis of the sera by ELISA indicated that the levels of different isotypes of anti-glycan Abs against specific glycan antigens was increased after influenza-infection, suggesting that the presentation of the glycan antigens and iso-type of the Abs are critical parameters to take into account when measuring anti-glycan Abs. This novel approach in avian influenza research may contribute to the development of a broad spectrum vaccine and improves our mechanistic understanding of innate and adaptive responses to glycans.
Keywords
N-Glycosylation sitesBPlNGSPAAHRPAIVCLRDC-SIGNMMRPolyacrylamidehaemagglutininimmunoglobulin Beta-propiolactoneHighly pathogenicDendritic cellsSialic acidC-type lectinlectinsDendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrinneuraminidaseavian influenza virusAntibodyHorseradish peroxidaseLow pathogenicMacrophage mannose receptor
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Authors
Eveline D. de Geus, Boris Tefsen, Daphne A. van Haarlem, Willem van Eden, Irma van Die, Lonneke Vervelde,