Aloe vera downregulates LPS-induced inflammatory cytokine production and expression of NLRP3 inflammasome in human macrophages

Article ID	Journal	Published Year	Pages	File Type
5916941	Molecular Immunology	2013	9 Pages	PDF

Abstract

Altogether, we show for the first time that Aloe vera-mediated strong reduction of IL-1Î² appears to be the consequence of the reduced expression of both pro-IL-1Î² as well as Nlrp3 inflammasome components via suppressing specific signal transduction pathways. Furthermore, we show that the expression of the ATP sensor P2X7 receptor is also downregulated by Aloe vera that could also contribute to the attenuated IL-1Î² cytokine secretion. These results may provide a new therapeutic approach to regulate inflammasome-mediated responses.

Keywords

Human acute monocytic leukemia cell line IL-1β NF-κB ERK MMP-9 RT-PCR ASC THP-1 GM-CSF Jnk P2X7R TLR4 IL-6 LPS IL-8 TNFα NLRP3 granulocyte-macrophage colony stimulating factor IkBα c-Jun N-terminal kinase PMA MAPK adenosine-5′-triphosphate ATP Aloe vera NLRP3 inflammasome Interleukin-8 interleukin-6 Interleukin-1β ELISA Enzyme-linked immunosorbent assay tumor necrosis factor-alpha Cytokine nuclear factor kappa-light-chain-enhancer of activated B cells nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha phorbol myristate acetate lipopolysaccharide matrix metallopeptidase 9 Macrophage Signal transduction apoptosis-associated speck-like protein containing a caspase recruitment domain real-time polymerase chain reaction mitogen-activated protein kinase extracellular-signal-regulated kinase Toll-like receptor 4