| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5917417 | Molecular Immunology | 2012 | 8 Pages |
Recent studies in rodents indicate that the nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-3 (NLRP3) inflammasome and a proinflammatory shift in the T cell population in adipose tissue (AT) contribute to AT inflammation and insulin resistance. We investigated: (1) the interplay between the NLRP3 inflammasome and T cell populations in abdominal subcutaneous AT in obese and lean humans in relation to AT inflammatory processes, and (2) involvement of the NLRP3 inflammasome and T cell populations in insulin resistance. Abdominal subcutaneous AT biopsies were collected in 10 obese men with impaired glucose tolerance and 9 lean normal glucose tolerant age-matched controls. AT gene expression of NLRP3 inflammasome-related genes and markers of T cell populations, chemoattraction, macrophage infiltration and other aspects of inflammation were examined. Furthermore, we examined systemic adaptive immune activation and insulin sensitivity (hyperinsulinemic-euglycemic clamp). CASPASE-1 mRNA and the proportion of Th1 transcripts (TBX21/CD3É) were significantly higher in AT from obese compared with lean subjects. CASPASE-1 expression and a relative increase in Th1 transcripts in AT were strongly associated with insulin resistance and impairments in glucose homeostasis. Gene expression of NLRP3, CASPASE-1, CD3É (pan T cells), TBX21 (Th1 cells) and RORC (Th17 cells) was positively, whereas GATA3 (Th2 cells) was inversely correlated with AT inflammation. Our data suggest that NLRP3 inflammasome activation and a Th1 shift in the T cell population in AT of obese subjects is related to insulin resistance and impaired glucose metabolism, which may be explained by AT inflammatory processes.
⺠Increased expression of CASPASE-1 in adipose tissue in human obesity. ⺠A Th1 shift in the T cell population in adipose tissue of obese humans. ⺠Interplay between the NLRP3 inflammasome and T cell populations in adipose tissue. ⺠NLRP3 inflammasome and a Th1 shift are linked to inflammation in humans. ⺠NLRP3 inflammasome and Th1 shift are associated with insulin resistance in humans.
