| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5917506 | Molecular Immunology | 2011 | 15 Pages |
Abstract
⺠We demonstrate a rapid LC-MS method for profiling IgG Fc modifications and allelic variants in blood. ⺠Information is obtained at the haplotype level. ⺠IgG1, but not IgG2, afucosylation is significantly increased in a small proportion of individuals. ⺠A new IgG1 allotype was identified. ⺠Correlations between allotypes and Fc modifications are amenable for study in large populations.
Keywords
GlcNAcL-1-tosylamido-2-phenylethyl chloromethyl ketoneTPCKTFAPQAADCCDTTFucoseFucPTMmAbAlleleantibody dependent cellular cytotoxicityMonoclonal antibodyEDTAEthylenediaminetetraacetic acidTrifluoroacetic acidpost-translational modificationdithiothreitollight chainheavy chainCoefficient of VariationScreeningreversed phaseFucosylationN-acetylglucosamineAllotypeGlycation
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Authors
Andrew M. Goetze, Zhongqi Zhang, Ling Liu, Frederick W. Jacobsen, Gregory C. Flynn,