Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
59411 | Chinese Journal of Catalysis | 2009 | 5 Pages |
Abstract
A novel application of hydrolase as catalyst for the addition of diethylzinc to aromatic aldehydes is reported. Optimum conditions were screened out. Under the optimum conditions (i.e., reaction at 40 °C using hyperthermophilice esterase APE1547, 4-Cl-benzaldehyde substrate, and trichloromethane solvent), this reaction provided optically active secondary alcohol with an enantiomeric excess up to 56% and a yield up to 78%. The possible mechanism was proposed based on experimental observations and molecular dynamics simulation. These observations extend the phenomenon of enzyme promiscuity.
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