The P-selectin gene Pro715 allele and low levels of soluble P-selectin are associated with reduced P2Y12 adenosine diphosphate receptor reactivity in clopidogrel-treated patients

ObjectiveTo investigate the interrelation between the P-selectin gene (SELP) Pro715 allele, P2Y12 adenosine diphosphate (ADP) receptor reactivity and levels of soluble P-selectin (sP-selectin) in clopidogrel treated patients.MethodsThe Pro715 allele within SELP was tested by allele specific PCR, sP-selectin was determined by ELISA, and P2Y12 receptor reactivity was analyzed by the VASP assay in 156 patients after angioplasty and stenting.ResultsCarriers of the SELP Pro715 allele had significantly lower levels of sP-selectin and P2Y12 receptor reactivity, and high on-treatment residual P2Y12 receptor reactivity (HRPR) was significantly less frequent compared to non-carriers (both p < 0.05). Further, patients within the lowest quartile of sP-selectin had significantly lower reactivity values and also less often HRPR compared to patients with higher sP-selectin (both p < 0.01).ConclusionThe SELP Pro715 allele is linked to low levels of sP-selectin, and both are associated with decreased P2Y12 ADP receptor reactivity in patients on clopidogrel therapy.