Article ID Journal Published Year Pages File Type
599573 Colloids and Surfaces B: Biointerfaces 2015 6 Pages PDF
Abstract

•We have developed a procedure for the purification of the magnetite gold core–shell nanoparticles.•We show that the enzyme activity decreases upon exposure of the NPs to the LF AC magnetic field.•Activity of selected enzymes can be efficiently manipulated by a remote magnetic field.•We demonstrate the effect of the spacer between NPs and enzyme on the kinetics of the enzyme reaction.

The possibility of remotely inducing a defined effect on NPs by means of electromagnetic radiation appears attractive. From a practical point of view, this effect opens horizons for remote control of drug release systems, as well as modulation of biochemical functions in cells. Gold-coated magnetite nanoparticles are perfect candidates for such application. Herein, we have successfully synthesized core–shell NPs having magnetite cores and gold shells modified with various sulphur containing ligands and developed a new, simple and robust procedure for the purification of the resulting nanoparticles. The carboxylic groups displayed at the surface of the NPs were utilized for NP conjugation with a model enzyme (ChT). In the present study, we report the effect of the low-frequency AC magnetic field on the catalytic activity of the immobilized ChT. We show that the enzyme activity decreases upon exposure of the NPs to the field.

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Related Topics
Physical Sciences and Engineering Chemical Engineering Colloid and Surface Chemistry
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