| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5996949 | Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health | 2013 | 4 Pages |
Abstract
Recent advances have been made in the study of urinary proteomics as a diagnostic tool for renal disease and pre-eclampsia which requires accurate measurement of urinary protein. We compared different protein assays (Bicinchoninic acid (BCA), Lowry and Bradford) against the 'gold standard' amino-acid assay in urine from 43 women (8 non-pregnant, 34 pregnant, including 8 with pre-eclampsia). BCA assay was superior to both Lowry and Bradford assays (Bland Altman bias: 0.08) compared to amino-acid assay, which performed particularly poorly at higher protein concentrations. These data highlight the need to use amino-acid or BCA assays for unprocessed urine protein estimation.
Keywords
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Authors
Hiten D. Mistry, Kate Bramham, Andrew J. Weston, Malcolm A. Ward, Andrew J. Thompson, Lucy C. Chappell,