| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 599865 | Colloids and Surfaces B: Biointerfaces | 2013 | 7 Pages |
•Organophosphorous compound modification of magnetic nanoparticles has been obtained.•Successful connection with trypsin via organophosphorous compounds has been tested.•Influence on modification from particles coating layer have been tested.
In this work magnetite nanoparticles covered by gold and silver shell were obtained. Analyzed particles were modified by two kinds of organophosphorous compounds: 3-phosphonopropionic acid and 16-phosphonohexadecanoic acid. Enzyme immobilization on particles modified in such a way was tested. The crystal structure of obtained nanoparticles was characterized by transmission electron microscopy and X-ray diffraction. Possible changes on the surfaces were analyzed by the use of infrared spectroscopy. Magnetic properties were studied by Mössbauer spectroscopy.
Graphical abstractThe magnetic nanoparticles are particularly interesting due to their potential application as a transfer media for the linkers which are specifically bonded to the surfaces. Usage of organophosphorous compounds allows to improve efficiency of magnetic separation of the hierarchical nanostructures.Figure optionsDownload full-size imageDownload as PowerPoint slide
