| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 600046 | Colloids and Surfaces B: Biointerfaces | 2013 | 8 Pages |
A novel electrochemical sensor for the simultaneous determination of glutathione (GSH) and nicotinamide adenine dinucleotide (NADH) is described. The sensor is based on a carbon paste electrode (CPE) modified with benzamide derivative and multiwall carbon nanotubes. This mixture makes a modified electrode that is sensitive for the electrochemical detection of these compounds. Under optimum conditions and at pH 7.0, oxidation of GSH occurs at a potential of about 330 mV less positive than that at an unmodified CPE. The voltammetric peak currents are linearly dependent on GSH and NADH concentrations in the ranges 0.09–300 μmol L−1 GSH and 5.0–600 μmol L−1 NADH. The detection limits found for GSH and NADH were 0.05 μmol L−1 and 1.0 μmol L−1, respectively. The electrochemical sensor was also used for the determination of GSH in urine, pharmaceutical and hemolysed erythrocyte samples.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► A new modified electrode for the determination of glutathione and NADH is described. ► Dinitrobenzamide derivative incorporated with MWCNTs used to prepare the electrode. ► Glutathione and NADH could be detect as low as 0.05 μmol L−1 and 1.0 μmol L−1. ► Simultaneous or independent measurements of the two analytes are possible.
