Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
600082 | Colloids and Surfaces B: Biointerfaces | 2013 | 6 Pages |
•We constructed a hE-cad–Fc fusion protein to modify hydrophobic materials.•We characterized the hE-cad–Fc fusion protein on a polystyrene plate.•The hE-cad–Fc matrix enhanced the adhesion and proliferation of hMSCs.•We preliminarily studied the molecular mechanism.
A fusion protein consisting of human E-cadherin extracellular domain and the immunoglobulin G Fc region (hE-cadherin–Fc) was prepared and used as a cell–cell adhesion biomimicking matrix for the in vitro expansion of human mesenchymal stem cells (hMSCs) for use in regenerative medicine. The hE-cadherin–Fc was stably immobilized onto a polystyrene plate due to the hydrophobicity of the Fc domain, enhancing the surface wettability and topography of the plate. The hE-cadherin–Fc matrix markedly promoted the cell adhesion and proliferation of hMSCs compared with the tissue culture-treated plate (TC-PS) and the gelatin-coated plate. Furthermore, the expanded hMSCs on the hE-cadherin–Fc were positive for CD105, similar to those from the gelatin. Additionally, the expression of E-cadherin and β-catenin in the hMSCs was improved on the hE-cadherin–Fc matrix, suggesting that the interactions of the hE-cadherin–Fc matrix with the hMSCs were substitutes for the cell–cell adhesion junctions during the initial culture stage in the absence of intercellular interactions. The hE-cadherin–Fc was shown to be a promising artificial ECM for the in vitro expansion of hMSCs.
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