| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 600141 | Colloids and Surfaces B: Biointerfaces | 2013 | 7 Pages |
This research is aimed to develop a kind of poly(ethylene glycol) shell-sheddable magnetic nanomicelle as the carrier of doxorubicin (Dox) in order to enhance its cellular uptake ability, and achieve synchronous magnetic resonance imaging (MRI)-visible function. Firstly, the five-member rings in poly (l-succinimide) (PSI) were successively opened by the amino terminated disulfide-linked poly(ethylene glycol) monomethyl ether (mPEG-SS-NH2) and dopamine (DA) to produce the graft copolymer of mPEG-SS-NH-graft-PAsp-DA. And then, drug-loaded magnetic nanomicelles of mPEG-SS-NH-graft-PAsp-DA@Fe3O4.Dox were obtained by the Fe3O4 nanoparticle-induced self-assembly of mPEG-SS-NH-graft-PAsp-DA. These magnetic nanomicelles showed spherical shapes with average particle size of about 120 nm measured by dynamic light scattering (DLS). Due to the detachment of PEG shell in the presence of dithiothreitol (DTT), the magnetic nanomicelles showed accelerated in vitro release of Dox, and enhanced cellular uptake ability. Compared with free Dox, the Dox-loaded magnetic nanomicelles showed essential decreased cytotoxicity against Bel-7402 cell line. If its high r2 relaxation rate (221 mM−1 s−1) and good negative contrast effect for magnetic resonance imaging (MRI) were taken into account, mPEG-SS-NH-graft-PAsp-DA@Fe3O4.Dox could be used as MRI-detectable drug carrier of Dox with enhanced cellular uptake ability.
Graphical abstract.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Magnetic nanomicelles as the carriers of doxorubicin were fabricated by Fe3O4 nanoparticle-induced self-assembly. ► The detachment of PEG in reduction environment resulted in significantly improved cellular uptake of nanomicells. ► The nanomicelles showed reduction-sensitive drug release profile. ► The nanomicelles showed decreased cytotoxicity against Bel-7402 cell compared with free doxorubicin and good in vitro MR imaging effect.
