| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 600251 | Colloids and Surfaces B: Biointerfaces | 2012 | 8 Pages |
In this paper, we report a feasible solvent-free approach for the synthesis and self-assembling of gold nanorods after bioconjugation to antibodies; scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images revealed a remarkable shape and size narrow distribution, as well as a tendency to form linear assemblies on a clean gold surface, upon interaction with antibodies.These bioconjugated gold nanorods were in turns tested in a model Fourier Transform Surface Plasmon Resonance (FT-SPR)-based immunosensor, leading to an improvement of the detection sensitivity by a factor of 8.The results highlight the simplicity of the synthesis protocol of gold nanorods and the interest of using them as labels to enhance the sensitivity of SPR-based sensors.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Synthesis protocol of bioconjugated gold nanorods with rIgG. ► Remarkable self-assembling of nanorods in linear chains via electrostatic interactions. ► Immobilization and characterization of anti-rIgG–BSA–rIgG system on gold surface previously functionalized by CYS/PDC. ► Enhance sensitivity of FT-SPR-based sensors.
