Article ID Journal Published Year Pages File Type
600656 Colloids and Surfaces B: Biointerfaces 2012 6 Pages PDF
Abstract

We investigate the intracellular uptake of 7.3 nm, 21.2 nm and 31.3 nm average size pure colloidal gold nanoparticles synthesized using femtosecond laser ablation technique in pure water. Dark-field imaging, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) was used to assess the uptake of these pure gold nanoparticles in the pancreatic tumor cell line. We show that these ligand-free gold nanoparticles are non-toxic to these cells. The nanoparticles and cell images indicated that unmodified gold nanoparticles interacted with the cells, despite negative surface charge on both the cells and the nanoparticles. We also demonstrate that the uptake of the gold nanoparticles is size-dependent.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Generation of gold nanoparticles in pure water using pulsed laser. ► Transmission electron microscopy of gold nanoparticles. ► Gold nanoparticles internalized in tumor cell.

Related Topics
Physical Sciences and Engineering Chemical Engineering Colloid and Surface Chemistry
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