| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 601000 | Colloids and Surfaces B: Biointerfaces | 2011 | 6 Pages |
A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol–gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide-N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol–gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Printable bio-ink to covalently immobilize protein onto sol–gel substrate. ► Bio-ink was prepared using heterobifunctional crosslinker to attach avidin to silane. ► Silane unit reacted with the surface to covalently immobilize avidin. ► Bio-ink was both gravure and inkjet printed. ► 3-Aminopropylsilane gave best results.
