| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 601140 | Colloids and Surfaces B: Biointerfaces | 2011 | 10 Pages |
Arg-Glu-Asp-Val (REDV) peptide with endothelial cells (ECs) selectivity was immobilized onto PEG based polymeric coating via the active p-nitrophenyloxycarbonyl group. The adhesion and proliferation of human umbilical vein endothelial cells (HUVECs) and human aortic smooth muscle cells (HASMCs) onto surface modified either by REDV end-tethered polyethylene glycol (PEG) or by the complex of free PEG and REDV were investigated to understand the synergic action of nonspecific resistance of PEG and specific recognitions of REDV. Cell culture results indicated that the surfaces end tethered by REDV peptide via PEG “spacer” (n = 1, 6, 10) exhibited slight EC selectivity and showed small difference between different lengths of PEG chain. Both separate-culture and co-culture of HUVECs and HASMCs indicated that the introducing of free PEG into REDV tethered surface inhibited HASMCs adhesion significantly and remained a high level of HUVECs growth. Furthermore, the surface with short free PEG chain (n = 6) was much more effective to enhance ECs selectivity than long EG chain (n = 23). The combination of nonspecific resistance of short free PEG and the ECs selectivity of REDV peptide presents much better ability to enhance the competitive adhesion of HUVECs over HASMCs.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideResearch highlights► Two kinds of PEG based biomimetic copolymers containing tail like chain structure with active terminal group and random copolymer structure with free PEG chain are prepared. ► The surface coatings with two different structures containing PEG and REDV peptide exhibit good hemocompatibility. ► The surfaces end tethered REDV via PEG “spacer” (n = 1, 6, 10) exhibit slight EC selectivity and show little difference between different lengths of PEG “spacer” (n = 1, 6, 10). ► The combination of nonspecific resistance of short free PEG chain and the ECs selectivity of REDV (P(EG)6BN–REDV) presents much better ability enhancing the competitive growth of HUVECs and keep good HUVECs morphology over HASMCs.
