A glucose biosensor based on chitosan–Prussian blue–multiwall carbon nanotubes–hollow PtCo nanochains formed by one-step electrodeposition

In this paper, a simple one-step electrodeposition method is described to fabricate chitosan–Prussian blue–multiwall carbon nanotubes–hollow PtCo nanochains (CS–PB–MWNTs–H-PtCo) film onto the gold electrode surface, then glucose oxidase (GOD) and Nafion were modified onto the film subsequently to fabricate a glucose biosensor. The morphologies and electrochemistry of the composite were investigated by using Fourier transform infrared (FTIR) spectrometry, scanning electron microscopy (SEM) and electrochemical techniques including cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), respectively. The performances of the biosensor have been investigated by chronoamperometry method under the optimized conditions. This biosensor showed a linear response to glucose range from 1.5 μM to 1.12 mM with a detection limit of 0.47 μM (S/N = 3), a high sensitivity of 23.4 μA mM−1 cm−2, and a fast response time. The apparent Michaelis–Menten constant (KMapp) was 1.89 mM. In addition, the biosensor also exhibited strong anti-interference ability, excellent stability and good reproducibility.

Graphical abstractThe schematic illustration of stepwise fabrication process of the glucose biosensors.Figure optionsDownload full-size imageDownload as PowerPoint slideResearch highlights► Use one-step electrodeposition to fabricate the CS–PB–MWNTs–H-PtCo composite. ► Research pH value, potential and deposition time on the biosensor performance. ► Compare the conductivity and performance of the different modified electrodes. ► Compare the performance of the biosensor use hollow and solid PtCo nanoparticles. ► Apply the prepared biosensor to detect glucose.