| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 601187 | Colloids and Surfaces B: Biointerfaces | 2011 | 6 Pages |
An electrochemical immunosensing assay for myeloperoxidase (MPO) determination in human serum has been developed. Firstly, l-Cysteine was initially electropolymerized on an Au electrode to form l-Cysteine film. After that cerium dioxide (CeO2) dispersed in 1-butyl-3-methylimidazolium hexafluorophosphate (BMIMPF6) were immobilized on the l-Cysteine film. Then the negatively charged nanogold particles were adsorbed onto the membrane via the positive charge of CeO2, which aimed at assembling more antibody of MPO (anti-MPO). The resulting immunosensor showed a high sensitivity, broad linear response to the MPO concentration comprised between 10 ng/mL and 400 ng/mL with a detection limit of 0.06 ng/mL. Moreover, the surface morphology of the electrode was studied by means of a scanning electron microscope and the electrochemical properties of the fabricated immunosensor were further characterized by cyclic voltammetry. Also, factors influencing the performance of the resulting immunosensors were studied in detail.
Graphical abstractHydrophobic BMIMPF6 with high conductivity can help avoid the spillage of the other materials modified on the electrode. The negatively charged nanogold particles were adsorbed onto the membrane via the positive charge of CeO2, which aimed at assembling more antibody of MPO.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Nanogold/nanoCeO2-BMIMPF6/l-Cysteine composite film modified Au electrode has been developed for the determination of myeloperoxidase. ► Adsorption of anti-MPO on nanogold/nanoCeO2-BMIMPF6/l-Cysteine film. ► Response mechanism of the developed immunosensor. ► For voltammetric determination of myeloperoxidase the detection limit was 0.06 ng/mL.
