| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 601303 | Colloids and Surfaces B: Biointerfaces | 2011 | 7 Pages |
A blood–brain barrier (BBB) model in vitro was established by cultivating human brain-microvascular endothelial cells (HBMECs) with the regulation of human astrocytes (HAs) (HBMEC/HA). Astrocyte-conditioned medium (ACM) was employed to constitute a confluent monolayer of HBMECs without directly conjugated HAs. HBMECs exhibited an orientated multiplication on the supporting membrane; while HAs grew in an overlapping fashion. In addition, HBMECs could propagate over the membrane pore, and the end-feet of HAs extended into the membrane pore to improve the integral feature of the BBB. HBMEC/HA demonstrated a high transendothelial electrical resistance (TEER) about 230 Ω cm2 and low permeability of propidium iodide (PI) about 4 × 10−6 cm/s. The order in TEER was HBMEC/HA > HBMECs with 100% ACM > HBMECs with 50% ACM > HBMECs. The reverse order was valid for the permeability of PI and uptake of calcein-AM by HBMECs. The tranwell culture of HBMECs and HAs displays appropriate characteristics of the BBB and can be applied to estimate the delivery efficiency of therapeutic chemicals for the brain-related disease.
Graphical abstract.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► We construct a blood–brain barrier (BBB) model comprising HA-regulated HBMECs (HBMEC/HA). ► HBMEC/HA displayed a high TEER and a low paracellular permeability. ► Astrocyte-conditioned medium (ACM) improves integrality of HBMECs for the BBB feature. ► HAs inhibits the uptake of calcein-AM by HBMECs.
