Electrochemical properties of myoglobin deposited on multi-walled carbon nanotube/ciprofloxacin film

We report the direct electrochemical and electrocatalytic properties of myoglobin (MB) on a multi-walled carbon nanotube/ciprofloxacin (MWCNT/CF) film-modified electrode. A highly homogeneous MWCNT thin-film was prepared on an electrode surface using ciprofloxacin (CF) as a dispersing agent. MB was then electrochemically deposited onto the MWCNT/CF-modified electrode. The MB/MWCNT/CF film was characterized by scanning electron microscopy and UV–visible spectroscopy (UV–vis). UV–vis spectra confirmed that MB retained its original state on the MWCNT/CF film. Direct electrochemical properties of MB on the MWCNT/CF film were investigated by cyclic voltammetry. The formal potential and electron transfer rate constant were evaluated in pH 7.2 buffer solution as −0.327 V and 300 s−1, respectively. In addition, the MB/MWCNT/CF-modified electrode showed excellent electrocatalytic properties for the reduction of hydrogen peroxide (H2O2). The MB/MWCNT/CF-modified electrode was used for the detection of H2O2 at concentrations from 1 × 10−6 M to 7 × 10−4 M in pH 7.2 buffer solution. Overall, the MB/MWCNT/CF-modified electrode was very stable and has potential for development as a H2O2 sensor.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideResearch highlights▶ Adsorption of myoglobin on carbon nanotube/ciprofloxacin film. ▶ Direct electron transfer of myoglobin and its bio-catalytic properties. ▶ Biosensing of H2O2 at myoglobin-carbon nanotube-ciprofloxacin film. ▶ Electrochemical and electrocatalytic properties of myoglobin.