Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6015900 | Epilepsy Research | 2013 | 5 Pages |
Abstract
The present study aimed to investigate whether Na+,K+-ATPase activity and phosphorylation state of the catalytic α subunit are altered by pentylenetetrazol (PTZ)-induced seizures. PTZ (30, 45 or 60 g/kg, i.p.) was administered to adult male Swiss mice, and Na+,K+-ATPase activity and phosphorylation state were measured in the cerebral cortex 15 min after PTZ administration. Na+,K+-ATPase activity significantly decreased after PTZ-induced seizures (60 mg/kg). Immunoreactivity of phosphorylated Ser943 at α subunit was increased after PTZ-induced seizures. A significant positive correlation between Na+,K+-ATPase activity and latency to myoclonic jerks and generalized seizures was found. Conversely, a strong negative correlation between Ser943 phosphorylation and latency to generalized seizures was detected. Given the role of Na+,K+-ATPase as a major regulator of brain excitability, Ser943 at Na+,K+-ATPase α subunit may represent a potentially valuable new target for drug development for seizure disorders.
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Authors
Bárbara P. Marquezan, VinÃcius R. Funck, Clarissa V. Oliveira, LetÃcia M. Pereira, StÃfani M. Araújo, Micheli S. Zarzecki, Luiz Fernando F. Royes, Ana Flávia Furian, Mauro S. Oliveira,