Prostaglandin E2 contributes to the synthesis of brain-derived neurotrophic factor in primary sensory neuron in ganglion explant cultures and in a neuropathic pain model

Article ID	Journal	Published Year	Pages	File Type
6018822	Experimental Neurology	2012	16 Pages	PDF

Abstract

âºCOX2 inhibitor or EP4 antagonist suppressed nerve injury increased BDNF in DRG neurons. âºBDNF expression is shifted to medium and larger size injured DRG neurons. âºIn DRG explant cultures, PGE2 increased BDNF levels. âºEP1 and EP4 receptors, PKA, ERK/MAPK and CREB signaling pathways were involved. âºInjured nerve-derived PGE2 contributes to BDNF up-regulation in DRG neurons following nerve injury.

Keywords

IL-6 NGF pKa HBSS cox2 CGRP PGE2 Fluorogold ERK NHS fluororuby Dulbecco Modified Eagle Medium dmPGE2 GAPDH PSNL DRG FBS CREB DMEM TRPV1 dorsal root ganglion BDNF MAPK Mitogen activated protein kinase kinase Small interfering RNA siRNA Nerve injury interleukin-6 Trk Neuropathic pain tract tracing fetal bovine serum normal horse serum cyclooxygenase 2 brain derived neurotrophic factor Sciatic nerve nerve growth factor Explant culture Substance P MEK Neurotrophin Inflammatory mediator transient receptor potential vanilloid-1 cAMP response element binding protein protein kinase A mitogen activated protein kinase Prostaglandin E2 calcitonin gene-related peptide Partial sciatic nerve ligation extracellular signal-regulated kinase glyceraldehyde-3-phosphate dehydrogenase tyrosine receptor kinase