Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6019294 | Experimental Neurology | 2010 | 10 Pages |
Abstract
This study investigates whether activation of p38 MAPK by the up-regulation of HSP 70 participates in the induction of brain ischemic tolerance by limb ischemic preconditioning (LIP). Western blot and immunohistochemical assays indicated that p38 MAPK activation occurred earlier than HSP 70 induction in the CA1 region of the hippocampus after LIP. P-p38 MAPK expression was up-regulated at 6Â h and reached its peak 12Â h after LIP, while HSP 70 expression was not significantly increased until 1Â day and peaked 2Â days after LIP. Neuropathological evaluation by thionin staining showed that quercetin (4Â ml/kg, 50Â mg/kg, intraperitoneal injection), an inhibitor of HSP 70, blocked the protective effect of LIP against delayed neuronal death that is normally induced by lethal brain ischemic insult, indicating that HSP 70 participates in the induction of brain ischemic tolerance by LIP. Furthermore, SB 203580, an inhibitor of HSP 70, inhibited HSP 70 activation in the CA1 region of the hippocampus induced by LIP either with or without the presence of subsequent brain ischemic insult. Based on the above results, it can be concluded that activation of p38 MAPK participates in the brain ischemic tolerance induced by LIP at least partly by the up-regulation of HSP 70 expression.
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Authors
Xiao-Cai Sun, Xiao-Hui Xian, Wen-Bin Li, Li Li, Cai-Zhen Yan, Qing-Jun Li, Min Zhang,