Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
602323 | Colloids and Surfaces B: Biointerfaces | 2009 | 8 Pages |
Abstract
Pullulan acetate (PA) was synthesized by the reaction of pullulan with acetic anhydride in the presence of pyridine. PA was characterized by Fourier transform infrared (FT-IR) and proton nuclear magnetic resonance (1H NMR). A solvent diffusion method was employed in the current work to prepare PA nanoparticles. This technique had some advantages compared with other methods. The particle size increased from 185.7Â nm to 423.0Â nm with the degree of acetylation increasing from 2.71 to 3.0. Drug-loaded PA nanopaticles were prepared for controlled release of epirubicin (EPI). The drug entrapment and drug content increased with the degree substitution of PA increasing. EPI was released from the nanoparticles in a biphasic profile with a fast release rate in the first 10Â h followed by a slow release in vitro. A higher cytotoxicity against KB cells was found for EPI-loaded PA nanoparticles in comparison with free EPI. Confocal laser scanning microscopy (CLSM) observations indicate that EPI-loaded nanoparticles were internalized and released in the cytoplasmic compartment.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Colloid and Surface Chemistry
Authors
Hui-zhu Zhang, Fu-ping Gao, Ling-rong Liu, Xue-min Li, Zhi-min Zhou, Xin-du Yang, Qi-qing Zhang,