Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
602367 | Colloids and Surfaces B: Biointerfaces | 2008 | 5 Pages |
Abstract
In prokaryotic cells, the hypothesis of the existence of lipid domains was considered. In order to test this hypothesis and study the organization of lipids in the inner membrane of Escherichia coli, we elaborated Langmuir films mimicking the inner leaflet of this membrane by considering lipids extracted from the inner membrane of E coli by Folch protocol.Lipid monolayers were elaborated by using these extracts (Langmuir technique); the organization of the resulting films was studied at the air–water interface by Brewster angle microscopy and after transfer onto muscovite by atomic force microscopy. The existence of domains was demonstrated for different interfacial pressures of biological interest, and their stability was studied.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Colloid and Surface Chemistry
Authors
Zakia Zerrouk, Stéphane Alexandre, Céline Lafontaine, Vic Norris, Jean-Marc Valleton,