Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
602456 | Colloids and Surfaces B: Biointerfaces | 2009 | 6 Pages |
Abstract
This investigation describes the surface characterization of rabbit immunoglobulin G (IgG) conjugated with gold nanoparticles. Goat anti-rabbit immunoglobulin G tagged with 5Â nm gold nanoparticles was applied to detect the IgG. Then, the autocatalyzed deposition of Au3+ onto the surface of anti-IgGAu increased the surface area per gold nanoparticle. The immobilization chemistries and the atomic concentrations of Au4f, P2p, S2p, C1s, N1s and O1s of the resulting antibody-modified Au electrodes were determined by X-ray photoelectron spectroscopy (XPS). The sulfur that is involved in the cysteamine binding and the enlargement of the gold nanoparticles are identified using cyclic voltammetry. The results reveal that the surface area per gold particle, following the autocatalyzed deposition Au3+ on the surface of anti-IgGAu, was approximately seven times higher than that before deposition.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Colloid and Surface Chemistry
Authors
Lee-Jene Lai, Yaw-Wen Yang, Yao-Kwang Lin, Li-Ling Huang, Yi-Heui Hsieh,