Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
602618 | Colloids and Surfaces B: Biointerfaces | 2007 | 4 Pages |
Abstract
Preparation of distributed virus on a solid substrate is a prerequisite for investigation of the properties and individualism of virus, while many previous studies showed that virus has a tendency to aggregate on solid substrates. In this communication, we report a novel approach by which well-separated recombinant adeno-associated virus serotype 2 (rAAV2) could be prepared on bare mica surface. The key technique in this approach is the addition of less than 3% (v/v) glycerol into the virus solution and subsequently deposition onto mica surface for the sample preparation. The possible mechanisms are also briefly discussed.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Colloid and Surface Chemistry
Authors
Peng Wang, Hai Li, Hai Jun Yang, Hua Bing Wang, Jun Hong Lü, Yi Zhang, Jun Hu,