Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
602712 | Colloids and Surfaces B: Biointerfaces | 2007 | 5 Pages |
Abstract
The expression vector, pBI121CTBVP1, containing the fusion of the foot and mouth disease virus (FMDV) VP1 gene and the cholera toxin B subunit (CTB) gene was constructed by fused PCR and transferred into potato (Solanum tuberosum L.) by Agrobacterium-mediated transformation. Transformed plants were obtained by selecting on kanamycin-resistant medium strictly and regenerated. The transgenic plantlets were identified by PCR, Southern-blot and the production of fused protein was confirmed and quantified by Western-blot and ELISA assays. The results showed that the fused genes were expressed stablely under the control of specific-tuber patatin promoter. The expressed fused proteins have a certain degree of immunogenicity.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Colloid and Surface Chemistry
Authors
Dong-Mei He, Kai-Xian Qian, Gui-Fang Shen, Yi-NĂ¼ Li, Zhi-Fang Zhang, Zhong-Liang Su, Hong-Bo Shao,