Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
602747 | Colloids and Surfaces B: Biointerfaces | 2008 | 8 Pages |
Abstract
S-layer proteins are commonly found in bacteria and archaea as two-dimensional monomolecular crystalline arrays as the outermost cell membrane component. These proteins have the unique property that following disruption by chemical agents, monomers of the protein can re-assemble to their original lattice structure. This unique property makes S-layers interesting for utilization in bio-nanotechnological applications. Here, we show that the addition of S-layer proteins to bilayer lipid membranes increases the lifetime and the stability of the bilayer. M2δ ion channels were functionally incorporated into these S-layer stabilized membranes and we were able to record their activity for up to 20 h. Transmission electron microscopy (TEM) was used to visualize the 2D crystalline pattern of the S-layer and the M2δ ion channel characteristics in bilayer lipid membrane's were compared in the presence and absence of S-layers
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Colloid and Surface Chemistry
Authors
Henk M. Keizer, Martin Andersson, Chris Chase, William P. Laratta, Josh B. Proemsey, Joel Tabb, Joanna R. Long, Randolph S. Duran,