Article ID Journal Published Year Pages File Type
602844 Colloids and Surfaces B: Biointerfaces 2006 10 Pages PDF
Abstract
Using a prepared computer program, the experimental data are properly fitted into a binomial exponential relation, considering a two-phase denaturation pathway including a kinetically stable folded intermediate formed at SDS concentration of 1.1 mM. Forward and backward rate constants are estimated as: k1 = 0.2141 ± 4.5 × 10−3, k2 = 5.173 × 10−3 ± 8.3 × 10−5, k−1 = 0.09432 ± 3.6 × 10−4 and k−2 = 2.079 × 10−3 ± 5.6 × 10−5 s−1 for the proposed mechanism. The rate-limiting step as well as the reaction coordinates in the denaturation mechanism are established. The mechanism involves formation of a kinetically stable folded native like intermediate through the electrostatic interactions. The intermediate was found to be more stable even than the native form (by about 9 kJ mol−1) and still hexamer, because no loss of amplitude was observed. Electrophoresis experiments on the native and surfactant/urease complexes indicated a higher mobility for the kinetically folded native like intermediate.
Related Topics
Physical Sciences and Engineering Chemical Engineering Colloid and Surface Chemistry
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