Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
602844 | Colloids and Surfaces B: Biointerfaces | 2006 | 10 Pages |
Abstract
Using a prepared computer program, the experimental data are properly fitted into a binomial exponential relation, considering a two-phase denaturation pathway including a kinetically stable folded intermediate formed at SDS concentration of 1.1 mM. Forward and backward rate constants are estimated as: k1 = 0.2141 ± 4.5 Ã 10â3, k2 = 5.173 Ã 10â3 ± 8.3 Ã 10â5, kâ1 = 0.09432 ± 3.6 Ã 10â4 and kâ2 = 2.079 Ã 10â3 ± 5.6 Ã 10â5 sâ1 for the proposed mechanism. The rate-limiting step as well as the reaction coordinates in the denaturation mechanism are established. The mechanism involves formation of a kinetically stable folded native like intermediate through the electrostatic interactions. The intermediate was found to be more stable even than the native form (by about 9 kJ molâ1) and still hexamer, because no loss of amplitude was observed. Electrophoresis experiments on the native and surfactant/urease complexes indicated a higher mobility for the kinetically folded native like intermediate.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Colloid and Surface Chemistry
Authors
K. Nazari, A. Mahmoudi, N. Esmaeili, L. Sadeghian, A.A. Moosavi-Movahedi, R. Khodafarin,