A system for studying the effect of mechanical stress on the elongation behavior of immobilized plant cells

The ability to apply controllable mechanical compressive force is essential for the study of plant cells responses to environmental stimulations. The work presented here aims towards establishing a system, which consists of a fabricated apparatus (including a loading unit, displacement sensor, data collector and processor, and a feedback control) and a protocol for test specimen preparation and force loading. By using a force–feedback control circuit coupled to a microchip, delivering the pre-defined and actual controlled stimulus is achieved. To calibrate the apparatus, the corresponding voltages are compared to the known weights. A linear regression is fit to the experimental data and a standardized coefficient of 0.998 is calculated. The morphological changes in response to mechanical stresses were investigated in agarose gel embedded chrysanthemum protoplasts, which tended to be elongated with a preferential axis oriented perpendicularly to the compressive stress direction. The results also indicated that there existed a certain dose-dependent relationship between the intensity of compressive force and the stress-induced responses. Additionally, the elongation response with preferential orientation was inhibited by application of RGD peptides, and its inverted sequence, DGR peptides failed to antagonize the effect of mechanical force on elongation performance.