In vivo detection of microscopic anisotropy using quadruple pulsed-field gradient (qPFG) diffusion MRI on a clinical scanner

► We develop a novel mPFG diffusion MRI sequence for clinical scanners. ► We apply mPFG MRI to detect microscopic anisotropy in the corpus callosum. ► The information measured with mPFG MRI is complementary to DTI-derived metrics. ► Integration with high-resolution DTI allows estimation of average axon diameters.